Document Type : Original Research Article


1 Department of Chemistry, Al-Qalam University, Katsina, Katsina State Nigeria

2 Department of Biological Sciences, Al-Qalam University, Katsina, Katsina State Nigeria



Antioxidant agents have been reported to play a significant role in protecting against infections induced by oxidant damage. Erythrophleum africanum have been found to be a reservoir of antioxidant agents. The powdered stem bark sample was macerated with acetone, methanol, and water successively for two weeks by using standard maceration method. The mixtures were agitated with a magnetic stirrer to dissolve the particles, decanted, filtered, and concentrated on rotavapor (R110) at 40  to obtain the acetone, methanol, and water (aqueous) extracts. By using hydrogen peroxide (H2O2) scavenging assay, the antioxidant potentials of Erythrophleum africanum stem bark extracts was investigated for its free radical scavenging property. The preliminary phytochemical screening of the extracts revealed the presence of seven (7) different secondary metabolites including: saponins, tannins, flavonoids, quinones, glycosides, phenols, and steroids. From the H2O2 scavenging assay, the acetone, methanol, and water (aqueous) extracts showed good dose-dependent free radical scavenging potentials. Both acetone and methanol extracts showed remarkable antioxidant capabilities compared with the standard (ascorbic acid), with the exception of the aqueous extract, which is lower than the standard (ascorbic acid, acetone, methanol, and water: 1.851, 2.412, 2,888, and 1.289).  The presence of different secondary metabolites in the stem bark extracts of Erythrophleum africanum proves the use of the plant in folkore medicine and confirm its use as antioxidant. 

Graphical Abstract

Phytochemical Screening and Evaluation of the Antioxidant Potentials of the Stem Bark Extracts of Erythrophleum Africanum (African Blackwood)


Main Subjects

[1] A.Y. Andlauer, Y. Furs, Oxidative Stress and Neurodegenerative Disorder. J. Biomed. Sci., 5 (1998) 401-414.
[2] A. Atawodi, Medicinal Plants and Traditional Medicine in Africa. John Wiley and Sons. Chichester, UK. 2005.
[3] J.P.M. Bruneton, Leguminosae Sub-family Caealpinioideae. Flora of Tropical East Africa, Milne- Redhead, E. and R. M. Polhill (Eds.). White Friars Press, London; 2009, 230-243.
[4] A.B. Daniyan, A. Kamanyi, M.S. Achang, N.B. Chunga-Anye, D. N’jamen, Anti-inflammatory and analgesic properties of the stem bark extracts of Erythrophleum suaveolens (caesalpiniaceae), Guillemin and Perrottet. J. Ethnopharmacology., 77 (2011) 137-141.
[5] A. Tukur, N.M. Musa, H.A. Bello and N.A. Sani, Determination of the Phytochemical Constituents and Antifungal Properties of Annona senegalensis Leaves (African Custard Apple). ChemSearch J., 11 (2020) 16-32.
[6] L. Hua, A. Hutchings, J. Van Staden, Screening of Zulu Medicinal Plants for Prostaglandin Synthesis Inhibitors. J. Ethnopharmacology., 52 (2005) 95-100.
[7] M.W. Iwu, A.R. Duncan, C.O. Okunji, New Antimicrobials of Plants Origin. Perspectives on New Crops and New Uses. J. Janick (Ed) ASHS Press, Alexandria, V.A., 1999, 457-462.
[8] K. Piette, T. Maruyamu, T. Takiguchi, K. Okuda and L. Takazoe , Studies of Antioxidant Activities of Plants Extracts. Bulletin of Tokyo Denral Collection., 32 (2000) 17-21.
[9] J.J. Ruch, S.J. Cheng, J.E. Klaunig, Prevention of Cytotoxicity and Inhibition of Intercellular Communication by Anti-oxidant Catechins Isolated from Chinese Green Tea, Carcinogen., 10 (1989) 1003-1008.
[10] A. Sofowora, Recent Trend in Research into Africa Medicinal Plants. J. Ethnopharmacology, 38 (1993) 209-214.
[11] W.A.R. Thompson, Healing Plants. A Modern Herbal. Mi Grew Hill Company, U.K, 1978, 6-10.
[12] I.S. Yong, J.V. Woodside, Antioxidant in Health and Diseases. J. Clinical Pathology, 54 (2001) 176-186.
[13] A. Tukur, N.M. Musa, H.A. Bello and N.A. Sani, Determination of the Phytochemical Constituents and Antifungal Properties of Annona senegalensis Leaves (African Custard Apple). ChemSearch J., 11 (2020) 16-32.
[14] J.M. Dalziel, Useful plants of tropical West Africa. Crown Agents London. 1999, 432-434.
[15] M.H. Jinju, African Traditional Medicine. A case study of Hausa Medicinal Plants and Therapy. Gaskiya Corpn. Ltd. Zaria. Nigeria 1990, 43-50.
[16] A. Sofowora Medicinal plants and Traditional Medicine in Africa, Spectrum Books Limited Nigeria. 2008, 10-17.
[17] J.M. Watt, M.G. Breyer–Brandwijk, Medicinal and Poisonous plants of Southern and Eastern Africa. E. S Living Stone Edinburgle publishers. (1963) 1046-1048.
[18] K.R. Brain-Tuner, The practical evaluation of phytochemistry pharmaceuticals Wright Science Chila Bright, (1975) 120-140.
[19] T. AbdulRazaq, M.M. Nasiru, T.A. Jimoh, D.H. James and O.B. Martha, Phytochemical Screening and the Effect of Methanolic Leaves Extract of Senna mimosoides on Inflammatory Stimulus Induced Leukocyte Mobilization (In-vivo), Ad. J. Chem. B, 4 (2022) 29-38.