Document Type : Original Research Article


1 Research Institute of Applied Science, Academic Center of Education, Culture, and Research (ACECR), Tehran, Iran

2 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Tehran, Iran

3 Research Institute of Applied Science, Academic Center of Education, Culture and Research (ACECR), Tehran, Iran

4 Department of Chemical Engineering, Mahshahr Branch, Islamic Azad University, Mahshahr, Iran


Today, two serious issues threaten human life, such as water pollution with hydrogen sulfide and the emerging corona disease. The special structure of hemoglobin of worms can remove hydrogen sulfide and carry a large amount of oxygen at the same time, and therefore, it is a suitable candidate for solving these two problems. Accordingly, the hemoglobin of Eisenia foetida worms was extracted by homogenization and several centrifuges and filters, and then the dimensions of hemoglobin were measured by dynamic light scattering technique, and the concentration and number of disulfide bonds in hemoglobin were investigated. The results showed that hemoglobin purity after the third stage of centrifugation and the second filtration was 56.2 and 91.3%, the measured diameter was 155.5 and 596.9 nm, the concentration of hemoglobin was 0.015 mol/liter, and the number of disulfide bonds was 120, respectively. According to the results of the examination, the extracted hemoglobin, having a large number of disulfide bonds, can react with hydrogen sulfide, and having very large dimensions, it is suitable for carrying a lot of oxygen.

Graphical Abstract

Extraction of Hemoglobin from Eisenia Foetida Worms


Main Subjects

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