Document Type : Original Research Article


1 libyan authority for scientific research, Tripoli, Libya

2 Chemistry Department, Faculty of Arts and Science Msallata, Elmergib University, Al-Khoms, Libya


The total phenolic, total antioxidant, ash, moisture, and metal contents of basil leaves collected within Msallata, Libya, were evaluated. To investigate the bioactive compounds, the basils were extracted using four solvents: water, ethanol, chloroform, and ethyl acetate. Phytochemical screening experiments revealed the presence of a variety of bioactive compounds in ethanolic and aqueous extracts, including coumarins, flavonoids, alkaloids, tannins, phenols, carbohydrates, and proteins. Folin-Ciocalteu method was used to determine the total phenolic content of basil ethanolic extract. Furthermore, antioxidant activities were measured using phosphomolybdenum method. The total antioxidant capacity of basil was determined to be (49.8 14.7 mg ascorbic acid equivalent/g dry weight), while the total phenols were 39.75 (mg gallic acid equivalent/g dry weight). Likewise after dry digestion, the macro- and micro-metals; i.e. potassium, sodium, calcium, magnesium, and phosphorus, iron, copper, and zinc, in basil leaves were determined using Flame Photometry and Atomic Absorption Spectrophotometry. The mineral composition revealed that potassium (58288.33 370.32 mg/kg) had the highest concentration, while zinc had the lowest (18.39 0.37 mg/kg). The results showed that basil aqueous extract had the highest yield (14.80%) and ethyl acetate extract had the lowest (2.41%). However, the moisture and ash contents were determined to be 16.00% and 15.31%, respectively. Finally, the basil extracts demonstrated the potential for use as health-promoting food ingredients.

Graphical Abstract

Evaluation of some Chemical and Biochemical Constituents in Ocimum Basilicum Available in Msallata City- Libya


Main Subjects

[1] F. P. Casuga, A. L. Castillo, M. J.-A. T. Corpuz, GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves. Asian Pacific Journal of Tropical Biomedicine, 6 (2016) 957–961.
[2] G. Velmurugan, S. P. Anand, GC-MS Analysis of bioactive compounds on ethanolic leaf extract of Phyllodium pulchellum L. Desv. International Journal of Pharmacognosy and Phytochemical Research, 9 (2017) 114-118.
[3] M. F. Mahomoodally, Traditional medicines in Africa: an appraisal of ten potent African medicinal plants. Evidence-based complementary and alternative medicine, 2013 (2013) 1-15.
[4] K. Nisha, M. Darshana, G. Madhu, M. K. Bhupendra, GC-MS analysis and anti-microbial activity of Psidium guajava (leaves) grown in Malva region of India. International Journal of Drug Development and Research, 3 (2011) 237-245.
[5] T. Starlin, P. S. Prabha, B. K. A. Thayakumar, V. K. Gopalakrishnan, Screening and GC-MS profiling of ethanolic extract of Tylophora pauciflora. Bioinformation, 15 (2019) 425-429.
[6] N. U. Olivia, U. C. Goodness, O. M. Obinna, Phytochemical profiling and GC-MS analysis of aqueous methanol fraction of Hibiscus asper leaves. Future Journal of Pharmaceutical Sciences, 7 (2021) 1-5.
[7] K. Klaper, J. A. Hammerl, J. Rau, Y. Pfeifer, G. Werner, Genome-based analysis of Klebsiella spp. isolates from animals and food products in Germany, 2013–2017. Pathogens, 10 (2021) 573.
[8] T. Yuliana T.  Yuliana's LiveDNA, T. Rialita, V. Widyaningtyas, F. Hayati, A.B. Setiawan and R. Safitri, Antimicrobial Effect of Basil Leaf Extract (Ocimum sanctum L.) to Preserve the Quality of Wet Noodles, Asian Journal of Plant Sciences, 2023, 22(1), 199-205.
[9] R. Singh,  N. GautamA. Mishra, R. Gupta, Heavy metals and living systems: An overview. Indian Journal of Pharmacology, 43 (2011), 246–253.
[10] C. Dinu, S. Gheorghe, A. G. Tenea, C. Stoica, G.-G. Vasile, R. L. Popescu, E. A. Serban, L. F. Pascu, Toxic Metals (As, Cd, Ni, Pb) Impact in the Most Common Medicinal Plant (Menthapiperita). International Journal of Environmental Research and Public Health, 18 (2021) 3904.
[11] J. Olowoyo, E. van Heerden, J. Fischer, C. Baker, Trace metals in soil and leaves of Jacaranda mimosifolia in Tshwane area, South Africa. Atmospheric Environment, 44 (2010) 1826–1830.
[12] B. Teofilovi´c, A. Tomas, N. Marti´c, N. Stilinovi´c, M. Popovi´c, I. ˇCapo, N. Gruji´c, B. Ilinˇci´c, A. Raˇskovi´c, Antioxidant and hepatoprotective potential of sweet basil (Ocimum basilicum L.) extract in acetaminophen-induced hepatotoxicity in rats, Journal of Functional Foods, 2021 (87) 104783.
[13] M. Suriyavanthana, M. Punithavanthi, Phytochemical analysis and antioxidant profile of Ocimum sanctum Linn. 5th International Conference on Emerging Trends in Engineering, Technology, Science and Management, Institution of Electronics and Telecommunications Engineers, Ganganagar, Bengaluru, Karnataka, 6 (2017) 1380- 1389.
[14] T. M. B Bandiola, Extraction and qualitative phytochemical screening of medicinal plants: a brief summary. International Journal of Pharmacy, 8 (2018) 137-143.
[15] M. H. Labiad, H. Harhar, A. Ghanimi, M. Tabyaoui, Phytochemical screening and antioxidant activity of Moroccan Thymus satureioïdes extracts. Journal of Materials and Environmental Sciences, 8 (2017) 2132-2139.
[16] Z. Najah, K. M. Elsherif, M. Alshtewi, H. Attorshi, Phytochemical profile and heavy metals contents of codium tomentosum and sargassum hornschuchi. Journal of Applicable Chemistry, 4 (2015) 1821-1827.
[17] Z. Najah, K. M. Elsherif, E. Kawan, N. Farah, 2015 Phytochemical screening and heavy metals contents of nicotiana glauca plant. International Journal of Pharmacy and Pharmaceutical Research, 4 (2015) 82-91.
[18] Z. Najah, K. Elsherif, Analytical and phytochemical studies on zizyphus lotus. European Journal of Biomedical and Pharmaceutical Sciences, 3 (2016) 574-577.
[19] L. R. Bradley, Moisture and total solids analysis. Food analysis, 4 (2010) 85-103.
[20] H. R. Nadeem, S. Akhtar, P. Sestili, T. Ismail, S. Neugart, M. Qamar, T. Esatbeyoglu, Toxicity, Antioxidant Activity, and Phytochemicals of Basil (Ocimum basilicum L.) Leaves Cultivated in Southern Punjab, Pakistan, Foods, 11 (2022) 1239.
[21] R. S. Phatak, A. S. Hendre, Total antioxidant capacity (TAC) of fresh leaves of Kalanchoe pinnata. Journal of Pharmacognosy and Phytochemistry, 2 (2014) 32-35.
[22] N. Timothy, M. Abubakar, E. T. Williams, Proximate, Elemental and Anti-Nutrients Composition of Pumpkin Seed (cucurbita maxima duch ex lam) Obtained from Duvu Mubi South Adamawa State, Nigeria. International Journal of Nutrition and Food Sciences, 9 (2020) 112-117.
[23] I. M. K. Al-aubadi, The Nutritional and chemical content of basil leaves Ocimum basilicum L. Journal of Biotechnology Research Center, 5 (2011) 67-74.
[24] A. N. Adham, Comparative extraction methods, phytochemical constituents, fluorescence analysis and HPLC validation of rosmarinic acid content in Mentha piperita, Mentha longifolia and Osimum basilicum. Journal of Pharmacognosy and Phytochemistry, 3 (2015) 130-139.
[25] V. N. Daniel, I. E. Daniang, N. D. Nimyel, Phytochemical analysis and mineral elements composition of Ocimum basilicum obtained in jos metropolis, plateau state, Nigeria. International Journal of Engineering and Technology- The International Journals of Engineering and Sciences, 11 (2011) 161-165.
[26] M. Azam, Saba Irshad, Phytochemical screening and antibacterial activities of essential oil, ethanolic and methanolic extracts of Ocimum basillicum L. Pakistan Journal of Biochemistry and Molecular Biology, 49 (2016) 36-39.
[27] L. Mousavi, R. M. Salleh, V. Murugaiyah, Phytochemical and bioactive compounds identification of Ocimum tenuiflorum leaves of methanol extract and its fraction with an anti-diabetic potential. International Journal of Food Properties, 21 (2018) 2390-2399.
[28] M. A. Soobrattee, V. S. Neergheen, A. Luximon-Ramma, O. I. Aruoma, T. Bahorun, Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutation Research/Fundamental and Molecular mechanisms of mutagenesis, 579 (2005) 200-213.
[29] L. Wang, C. L. Weller, Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology, 17 (2006) 300-312.
[30] O. C. Adebooye, R. Vijayalakshmi, V. Singh, Peroxidase activity, chlorophylls and antioxidant profile of two leaf vegetables (Solanum nigrum L. and Amaranthus cruentus L.) under six pretreatment methods before cooking. International journal of food science & technology, 43 (2008) 173-178.
[31] G.-C. Yen, D.-Y. Chuang, Antioxidant properties of water extracts from Cassia tora L. in relation to the degree of roasting. Journal of agricultural and food chemistry, 48 (2000) 2760-2765.
[32] S. C. M. Burri, A. Ekholm, Å. Håkansson, E. Tornberg, K. Rumpunen, Antioxidant capacity and major phenol compounds of horticultural plant materials not usually used. Journal of functional foods, 38 (2017) 119-127.
[33] Y. A. A. Aburigal, M. E. S. Mirghani, E. Y. Elmogtaba, A. A. M. Sirible, N. B. Hamza, I. H. Hussein, Total phenolic content and antioxidant capacity of basil (Ocimum basilicum L.) leaves from different locations, International Food Research Journal, 24(Suppl) (2017) S378-S381.
[34] V. Katalinic, M. Milos, M. Jukic, M., Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols, Food Chemistry, 94 (2006) 550–557.
[35] A. F. Ahmed, A. K. Fatma A.K., Attia, Z. Liu, C. Li, J. Wei, W. Kang, Antioxidant activity and total phenolic content of essential oils and extracts of sweet basil (Ocimum basilicum L.) plants, Food Science and Human Wellness, 8(3) (2019) 299-305.
[36] S. Kumar, R. Sandhir, S. Ojha, Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Research Notes, 7 (2014) 1-9.
[37] P. Prieto, M. Pineda, M. Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry, 269 (1999) 337-341.
[38] S. Albayrak, A. Aksoy, S. Albayrak, O. Sagdic, In vitro antioxidant and antimicrobial activity of some Lamiaceae species. Iranian Journal of Science and Technology (Sciences), 37 (2013) 1-9.
[39] C. O. Akoto, A. Acheampong, Y. D. Boakye, A. A. Naazo, D. H. Adomah, Anti-inflammatory, antioxidant, and anthelmintic activities of Ocimum basilicum (Sweet Basil) fruits. Journal of Chemistry, 2020 (2020) 1-9.
[40] R. Dghaim, S. Al Khatib, H. Rasool, M. A. Khan, Determination of Heavy Metals Concentration in Traditional Herbs Commonly Consumed in the United Arab Emirates, Journal of Environmental and Public Health, 2015 (2015) 973878
[41] V. Jena, S. Gupta, Study of Heavy Metal Distribution in Medicinal Plant Basil, Journal of Environmental Analalytical Toxicology, 2 (2012) 161.
[42] I. Ałtyn, M. Twarużek, Heavy metal and mould contamination of herbal medicinal products – an overview, Annales Universitatis Paedagogicae Cracoviensis Studia Naturae, 3 (2018) 125-134
[43] Z. Javari, H. A. Asadi-Gharneh, Investigation of mineral nutrition of some basil (Ocimum basilicum L.) landraces grown in greenhouse condition, Research on Crops, 18(4) (2017) 768
[44] N. K. Fageria, A. Moreira, The role of mineral nutrition on root growth of crop plants. Advances in agronomy, 110 (2011) 251-331.
[45] R. Gyana, S. Sahoo, Role of iron in plant growth and metabolism. Reviews in Agricultural Science, 3 (2015) 1-24.