Document Type : Original Research Article
1 Chemistry Department, Al-Qalam University Katsina, Katsina State Nigeria. P.M.B 2137, IBB way Dutsinma Road, Katsina State Nigeria.
2 Chemistry Department, Ahmadu Bello University, Zaria, Kaduna State, Nigeria P.M.B 1044 Samaru, Zaria, Kaduna State, Nigeria
The secondary metabolite isolated from plant has been the main source of novel drugs which bears valuable therapeutic activities such as anthelmintic, antimicrobial, and antioxidant activities. Indigofera diphylla a semi–woody herb of dry sandy area belongs to the family fabaceae. The plant is being used as antidotes. The study was aimed at determining the chemical constituent of the extract in ethyl acetate of the root of Indigofera diphylla utilizing GC-MS. The plant was harvested and dried by air after that the root was separated and processed into powder. To obtain ethyl acetate extract, extraction by maceration was used. The Agilent GC-MS machine was utilized to analyze the chemical composition. There were found to be forty-five (45) different compounds from the ethyl acetate extract of root of Indigofera diphylla, among which 2-isopropoxyethyl propionate showed the highest peak area of 17.12% and 1-nitro-2-acetamido-1,2-dideoxy-d-mannitol showed the lowest peak area of 0.028%. The major identified compounds were methenamine, 5-fluoro-6-methyl-5-hepten-2-one, 2-(4-nitrobenzylideneamino)thiazole, hexadecanoic acid, 1,1-dimethylester, and n-hexadecanoic acid. The phytochemical result revealed that glycoside, saponins, and tannins were present. The phytochemical identified from the ethyl acetate extract of Indigofera diphylla may contribute to the plant's medical effectiveness and is a potential source of therapeutic medications.