Document Type : Original Research Article


1 Chemistry Department, Al-Qalam University Katsina, Katsina State Nigeria. P.M.B 2137, IBB way Dutsinma Road, Katsina State Nigeria.

2 Chemistry Department, Ahmadu Bello University, Zaria, Kaduna State, Nigeria P.M.B 1044 Samaru, Zaria, Kaduna State, Nigeria


The secondary metabolite isolated from plant has been the main source of novel drugs which bears valuable therapeutic activities such as anthelmintic, antimicrobial, and antioxidant activities. Indigofera diphylla a semi–woody herb of dry sandy area belongs to the family fabaceae. The plant is being used as antidotes. The study was aimed at determining the chemical constituent of the extract in ethyl acetate of the root of Indigofera diphylla utilizing GC-MS. The plant was harvested and dried by air after that the root was separated and processed into powder. To obtain ethyl acetate extract, extraction by maceration was used. The Agilent GC-MS machine was utilized to analyze the chemical composition. There were found to be forty-five (45) different compounds from the ethyl acetate extract of root of Indigofera diphylla, among which 2-isopropoxyethyl propionate showed the highest peak area of 17.12% and 1-nitro-2-acetamido-1,2-dideoxy-d-mannitol showed the lowest peak area of 0.028%. The major identified compounds were methenamine, 5-fluoro-6-methyl-5-hepten-2-one, 2-(4-nitrobenzylideneamino)thiazole, hexadecanoic acid, 1,1-dimethylester, and n-hexadecanoic acid. The phytochemical result revealed that glycoside, saponins, and tannins were present.  The phytochemical identified from the ethyl acetate extract of Indigofera diphylla may contribute to the plant's medical effectiveness and is a potential source of therapeutic medications.

Graphical Abstract

Phytochemical and Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Ethyl Acetate Root Extract of Indigofera Diphylla


Main Subjects

‎[1]         N.M. Saraswathi, M. Karthikeyan, S. Rajasekar, V. Gopal. Indigofera tinctoria Linn-‎A Phytopharmacological Review., 3 (2012) 164–9.
‎[2] ‎        H. Tapsoba, J. Deschamps. Use of medicinal plants for the treatment of oral diseases in ‎Burkina Faso., 104 (2006) 68–78. ‎
‎[3] ‎        A.I. Achika, R.G. Ayo, A.O. Oyewale, J.D. Habila. Flavonoids with ‎antibacterial and antioxidant potentials from the stem bark of Uapaca heudelotti. ‎Heliyon., 6 (‎2020‎) e03381. ‎‎
‎[4] ‎        H. Ibrahim, A.J. Uttu, M.S. Sallau, O.R.A. Iyun. Gas chromatography–mass ‎spectrometry (GC–MS) analysis of ethyl acetate root bark extract of Strychnos innocua ‎‎(Delile). Beni-Suef Univ J Basic Appl Sci., 10 (‎2021‎) 1–8. Available from: ‎‎
‎[5] ‎        F. Ahmad. Encyclopedia of Tropical Plants Identification and Cultivation of over 3000 ‎Tropical plants. Firefly Books Ltd, New Zealand; 2011. ‎
‎[6] ‎        H.M. Burkill. The Useful Plants of Tropical Africa. 2nd ed. Royal Botanic Gardens, ‎Kew, Richmond, United Kindom; (1995) 857
‎[7]‎ M.M. Mainasara, B.L. Aliero, S.S. Dahiru. Phytochemical and Antibacterial ‎Properties of Calotropis procera (Sodom apple) fruit and bark extracts. International Journal of Modern Botany, 1 (‎2011‎) 8-11.‎
‎[8]‎ C.F. Ukpabi, K.N. Agbafor, O.K. Ndukwe, A. Agwu, S.N. Nwachukwu. ‎Phytochemical Composition of Costus afer extract and its alleviation of carbon ‎tetrachloride induced hepatic oxidative strass and toxicity. International Journal of Botany., 2 (‎2012‎) 120-126.‎
‎[9]‎ A. Zafilazi, A. Andriasimahavandy, R.H. Randrinarivo, D.J. Ramaojisoa and M. ‎Andrianairivelo. Ethnobotanical, Antimicrobial and Phytochemical Screening of ‎Eurphorbia thymofila to cure the menstrual haemorrhage of women in Sakalava ‎Bernazava in the Northern Region of Madagascar. International Journal of Plant Research., 8 (‎2018‎) 1-7‎
‎[10] ‎     F.H.O. Al-Khaikani. Letter to Editor: The Forgotten Role of Methenamine to Prevent ‎Recurrent Urinary Tract Infection: Urgency for Reuse 100 Years After Discovery. ‎Pharm Biomed Res., 6 (‎2020‎) 247–50. ‎
‎[11] ‎     M.J. Kadhim, A.F. Al-rubaye, I.H. Hameed. Determination of Bioactive Compounds ‎of Methanolic Extract of Vitis vinifera Using GC-MS. International Journal of Toxicological and Pharmacological Research. 9 (‎2017‎) 113–26. ‎
‎[12] ‎     G.N. Ross, H.M. Fales, H.A. Lloyd, T. Jones, E. A. Sokoloski, K. Marshall-Batty, et al. Novel chemistry of abdominal defensive glands of nymphalid butterfly Agraulis ‎vanillae. J Chem Ecol., 27 (‎2001‎) 1219–28. ‎
‎[13] ‎     A. Petrou, M. Fesatidou, A. Geronikaki. Thiazole Ring-A Biologically Active ‎Scaffold. Molecules.  26 (2021) 3166.
‎[14] ‎     V. Aparna, K.V. Dileep, P.K. Mandal, P. Karthe, C. Sadasivan, M. Haridas  Anti-‎Inflammatory Property of n-Hexadecanoic Acid: Structural Evidence and Kinetic ‎Assessment. Chem Biol Drug Des., 80 (‎2012‎) 434–9. ‎