Nanotechnology is a swiftly growing field due to its huge range of uses in various branches of science and technology. Divergent types of routes are employed for the production of nanoparticles (NPs) because of their broad applications. The conventional physical and chemical approaches have certain constraints with them either in the form of chemical contaminations during their syntheses methodology or use of higher amount of energy. During the last one or two decades research have been concentrated on creating facile, safe, non-noxious, affordable and eco-accommodating procedures for synthesis of NPs. In order to get this purpose, green synthesis approaches have been improved in order to fill this lacuna. The biogenic synthesis of NPs is facile, one pot, eco-benevolent, sustainable and a green methodology. The different biological specimens like plant tissues, yeast, bacteria, fungi, etc. are used for green synthesis for metal oxide NPs. In this review, we summed up recent literature on biomimetic synthesis of silver oxide (Ag2O) NPs which have revolutionized method of fabrication for their stupendous applications in various sectors. Due to biocompatibility of Ag2O NPs, it has found its efficacious applications in biomedical field. The characterization techniques and mechanism of green synthesis of Ag2O NPs along with diverse applications have also been investigated.